Discovery in Practice · Reference library
Alpha assays in microplate assays
Explore alpha assays through measurement principles, assay guidance and practical observations. Follow the questions that match your experiment.
3 articles
Plate handling & assay conditions
Plate layout, timing and temperature influence what reaches the detector. Explore practical ways to understand those effects in microplate experiments.
Your Alpha assay is also a thermometer
Troubleshoot temperature effects in Alpha assays by testing liquid temperature, plate timing and control behavior under realistic screening conditions.
Why does my plate reader warm the plate when incubation is switched off?
Understand plate-reader warming, room-temperature chamber control and practical temperature checks for CellTiter-Glo and Alpha assays.
Why do Alpha or glow-luminescence controls drift from the first plate to the last?
Separate assay age, reagent changes and temperature drift when Alpha or glow-luminescence controls change from the first plate to the last.