Notes from the discovery benchMicroplate assays, readers & drug discovery
Discovery in Practice

Discovery in Practice · Reference library

BRET in microplate assays

Explore bret through measurement principles, assay guidance and practical observations. Follow the questions that match your experiment.

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Binding & proximity assays

Choose a readout that fits the interaction you want to study. Explore tracer design, proximity measurements and the conditions that affect apparent binding.

  1. NanoBRET: proximity needs a good alibi

    Improve NanoBRET assays with expression controls, simultaneous dual-emission detection, linearity checks and stable temperature. Includes worked examples.

    Assays · Published
    Plate effectsBRET

  2. How should I optimize NanoBRET donor and acceptor filters?

    Choose assay-specific NanoBRET emission filters using raw channels, negative controls, ratio precision and simultaneous dual-emission detection.

    Assays · Published
    BRET

By research topic

LuminescenceFluorescenceTR-FRET & HTRFPlate effectsAbsorbanceBRETBinding assaysScreening equipment