DNA quantification: five methods, five different answers
DNA mass, target copies, amplifiable library molecules and fragment integrity are different measurements. Choose the answer your next experiment needs before choosing the instrument.
CXComparisons
Choose the measurement.
Then choose the tools.
Assay formats and screening instruments, compared with the controls, conditions, and trade-offs that change a decision.
Assay comparisons
Microplate kinase assays: choose the event you need to measure
ADP production, ATP depletion, substrate phosphorylation and target binding are useful measurements. They are not interchangeable evidence of kinase inhibition.
Equipment comparisons
Automated confocal screening: compare the result, not the camera speed
Six representative high-content imaging families compared by optics, simultaneous channels, live-cell conditions and the work required to turn a plate into reliable measurements.