What does the spatial pattern of wells tell me?
Inspect spatial patterns alongside control statistics, then use those patterns to choose a follow-up check.
Discovery in Practice · Reference library
Plate effects
Plate maps, temperature, mixing and the spatial patterns behind assay results.
Start with the raw well positions. Use assay controls to distinguish plate handling and detection effects from the biological change you are testing.
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Does less CellTiter-Glo light mean fewer cells?
CellTiter-Glo measures ATP-dependent light, not cell number directly, so metabolism and detection conditions can change the signal independently of abundance. The article separates biological effects from reagent, temperature, mixing, and detector effects and proposes controls for each.
Did kinase activity change, or did ADP-Glo detection change?
ADP-Glo light is the endpoint of a multistep detection sequence, so a lower signal can arise outside the kinase reaction. This article shows how to reconstruct the endpoint with ATP/ADP controls and interpret temperature, timing, conversion, and ATP-dependent potency.